| Oxidative stress (OS)
is considered now as important summarizing pathogenetic section of
various cardiovascular, nervous, lung, eye, blood diseases, ageing
Thus monitoring of human organism oxidant/antioxidant status is urgent.
Antioxidant content in biological liquid – summary antioxidant activity
of the investigated object (AOA) could be evaluated by integral methods,
for example, by generally recognized spectrophotometric method TAS
(Randox). These methods, as a rule, are based on interactions of long
living free radicals which serve as a model of free radicals forming
in a living cell. But it is worth mentioning that the behaviour of
long and short living radicals in vivo might differ greatly. Providing
information on the sample AOA, the methods mentioned are characterized
with some peculiarities which limit their wide application.
- there are several stages of analysis taking rather long time;
- analytical signal is registered by expensive spectrophotometric
or fluorometric equipment,
- application of expensive reagents and high qualified staff is
Thus, the development of reliable, available, cheap method of antioxidant
stress monitoring in clinical conditions is very timely.
Interaction of antioxidants (AO) with free radicals (FR) and active
oxygen forms in water media is followed by electron transfer and,
therefore, is of electrochemical nature. Thus, it is worth while
to apply electrochemical methods for evaluation of integral AOA,
taking into consideration that these methods are characterized with
high sensitivity, simplicity and cost attractiveness; moreover,
they are available both in the laboratory and field conditions.
Potentiometric method of AOA evaluation with the use of mediator
systems was proposed by us earlier (Method of solution oxidant/antioxidant
activity determination, patent # 2235998, Russia).
- development of the techniques of AOA determination of blood
and erythrocyte mass with the aim of AOA cell component selection;
- development of the technique of AOA determination of seminal
fluid and methods of express diagnostics of the human reproductive
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